Intoduction to culture media and types

Culture media:


Most laboratoris purchase their media in a dehydrated form or reday to use.
Many culture media have generic name and and should not be assumed to be identical as the individual manufacture ingredients have unique characteistics.
Some culture media have proprietary names and is available only from particular companies.
Though can be purchased from others 'equivalent " items should not be considered to be identical.

Only two commercial available media are rigorously controlled to meet external regulatory specifications.
Standard Plate Agar and mueller-hinton agar.

General purpose media
These are non selective media that are designed to supportthe growth of ide variety of heterotrophic bacteria
This media contains ezyme or acid digests of animal or plant proteins as prime sources of organic carbon and nitrogen
Metal ions, vitamins, minerals re provided by yeast extracts / meat extracts.
The groth of more fastidious organisims is encouraged by growth promoting supplements.
Growth promoting supplements include horse/ sheep blood, Fides extract, Menadione/vitK, Ferric/Ferrous salts, vitamins, Horse/Rabbit serum, Yeast Autolysate, Sodium Pyruvate.

Selective isolation media.
This media is used for the isolation of certain micro-organisms and contains selective chemicals that supress the growth of others.

Usually the suppresed groth is during the early hrs of incubation which allos the slower variety to grow which are the unsuspected organisms.

Transport media
Semisolid media with/ without charcoal and antibiotics intended to keep delicate microbes alive during transit.

Antibiotic sensitivity media
Mueller-Hinton is the most widely used AST media. Isosensitivity and Direct Sensitivity test agars are used fro disc diffusion test or antibody dilution.

Sterility test
For products containing presevatives: Cluasen medium, Thioglycollate media.
For products not containing presevatives: Any non- selective broth medium.




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